Liquid process chromatography with LEWA systems

Purification (downstream processing) of materials by means of chromatography and other unit operations is crucial when manufacturing fine chemicals and active pharmaceutical ingredients.

Whether diaphragm metering pumps, metering stations or gradient mixers, at LEWA, we configure products according to your requirements. Our systems feature very high reproducible metering accuracy and large adjustment ranges, which means high product quality. These attributes have been proven in production on an industrial scale.

Your individual solution for downstream processing

LEWA delivers diaphragm metering pumps and complete metering systems to handle all types of downstream processing. Examples include gradient mixers for producing gradients and customized systems for inline dilution of buffers as well as metering stations. 


These solutions provide the following advantages:

•    Precise flow rates (incrementally or linearly variable, 1-99%)
•    Reproducible and highly accurate positioning (0.5%)
•    Hermetically tight system concept in downstream processing
•    Very large reproducible adjustment range of the pump/system
•    High availability, reliability and process safety
•    Reliable for validation thanks to high precision in downstream processing
•    Ability for inline cleaning and damping (CIP/SIP), optionally ventable
•    Hygienic pumps available (wetted electropolished, ASME-BPE certified)
•    Versions available for installation in clean rooms
•    Design as a complete skid with all process-relevant attachments for integration into chromatography systems is possible
•    Comprehensive documentation for qualification and validation
•    IQ/OQ/PQ support
•    Ex protection at the request of the customer

Areas of application: LEWA metering pumps, metering systems and gradient mixers are used in downstream processing, including in the following areas:

Low- and medium-pressure chromatography, for example

•    Preparative liquid column chromatography
•    Normal- and reversed-phase chromatography
•    Ion-exchange chromatography
•    Affinity chromatography, etc.
•    Downstream processing for purifying vaccines, innovative therapeutic proteins, monoclonal antibodies, hormones and blood plasma products

High-pressure chromatography

•    High-pressure liquid chromatography (HPLC)
•    Preparative countercurrent chromatography with supercritical CO2 using SFC (supercritical fluid chromatography)

Get in touch today so that we can talk about a strategy for implementing your downstream processing requirements!

Background: Process description of downstream processing

Liquid chromatography is the most important part of the purification process and provides a broad range of physical downstream processing methods for separating complex mixtures of substances.

The components to be separated are divided into two phases: 
The stationary phase in a column and the mobile phase, which flows through the stationary packing (here, liquid).

How does chromatography work?

A mixture of various components of a liquid/gas enter a process of process chromatography. The goal is to purify the mixture (downstream processing). The various separating and chromatography solutions are pumped through the system at various speeds. The interaction between the stationary and mobile phases in the system varies in intensity, causing the mixture of substances to separate. The lower the affinity of a molecule for the stationary phase is, the shorter the time in a chromatography column is, and vice versa.

Why is chromatography so special and important?

The chemical and bioprocess industries have the need to separate one product from a complex mixture and to eliminate impurities. This is an important step in the entire processing chain (downstream processing / cleaning) to produce a product with the required level of purity. Today there exists a broad range of methods that the industry can use to achieve these goals. For many reasons, chromatography is a very special and selective separation method that has been used for decades—primarily in the area of production. First, it can separate complex mixtures with great precision. Even very complicated molecules such as proteins, for instance, can be carefully separated from each other using chromatography. This way, basically any soluble or volatile substance can be purified if the correct adsorbents, carrier fluids / mobile phases are used and provided that process parameters are adapted and monitored (temperature, pH value, flow velocity, conductivity and much more).

Downstream processing is perfect for separating or purifying sensitive products from the pharmaceutical sector specifically.

Products

LEWA ecoflow diaphragm metering pump

LEWA ecoflow diaphragm metering pump

The ecoflow® diaphragm metering pump from LEWA is a true all-around talent in the field of metering and mixing and can be used in all industries.

LEWA ecoflow sanitary/hygienic

LEWA ecoflow sanitary food pump

The ecoflow® diaphragm metering pump in sanitary/hygienic design is the ideal pump for food and pharmaceutical products.

LEWA ecodos sanitary/hygienic

LEWA ecodos food pump

The LEWA ecodos® diaphragm metering pump with its sanitary/hygienic design is the ideal pump for food and pharmaceutical products in the low-pressure range.

LEWA intellidrive diaphragm metering pumps

LEWA intellidrive diaphragm metering pump

Use servomotor-controlled diaphragm metering pumps for precision metering and mixing of fluids in metering plants and systems.

LEWA FC laboratory pump

LEWA micrometering pump FC

The smallest flow rates and precise metering make LEWA micro-metering pumps of the FC series the first choice for laboratory pumps.

LEWA ecoflow process diaphragm pump

LEWA high pressure pump for process engineering

The LEWA ecoflow process diaphragm pump is the top performer among innovative and universal process pumps for high-pressure process technology.

Pomac rotary lobe pump

Pomac rotary lobe pump PLP

The rotary lobe pump – the solid endurance test vehicle from Pomac is used in nearly all industrial areas and can withstand continuous loads.

Customized production units

LEWA metering and mixing system

LEWA develops complete systems for metering, mixing, and filling fluids. Contact us and let us advise you!

Customer-specific metering systems

LEWA metering system

LEWA provides you with custom-designed metering systems according to your specifications. Put your trust in the experience gained from 60 years of metering technology in various industries.

Chemineer Mixing technology

Chemineer agitators

Chemineer agitators cover a wide range of products. From turbine agitators to impellers, these agitators can be used in almost any process that requires liquid or gas agitation.

Contact person

  • Sales Office Hungary
  • LEWA NIKKISO Austria GmbH - Hungarian Representative
  • +36 23 880-975
  • info@lewa.hu

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